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Image Search Results
Journal: Clinical and Experimental Immunology
Article Title: Human papillomavirus 16-specific T cell responses in classic HPV-related vulvar intra-epithelial neoplasia. Determination of strongly immunogenic regions from E6 and E7 proteins
doi: 10.1111/j.1365-2249.2009.04006.x
Figure Lengend Snippet: Ex vivo enzyme-linked immunospot–interferon-γ (ELISPOT–IFN-γ) responses in the three responder patients. Ex vivo ELISPOT–IFN-γ was performed as described in Materials and methods using peripheral blood mononuclear cells (PBMC) (4·105 cells/well) incubated with different peptides from E6 and E7 proteins (Table 2). The number of spots was calculated per million of PBMC.
Article Snippet: Briefly, nitrocellulose plates (Multi-Screen HA; Millipore, Bedford, MA, USA) were coated overnight at +4°C with 0·1 µg of
Techniques: Ex Vivo, Enzyme-linked Immunospot, Incubation